• Large monotub refill kit with 5lb bag of sterile milo grain and 5lb bag of Sterilized substrate
  • Large Monotub Refill Kit
  • 5-pound bag of milo (sorghum) grain in Unicorn bag
  • Shot of a 5 lb bag of not so simple substrate, mushroom substrate
  • Close-up of barehands holding moist CVG substrate block, highlighting texture of coco‑coir, vermiculite, gypsum blend.
1 5

Mycologysimplified

Large Monotub Refill Kit – Sterile Grain Spawn & CVG Substrate

Large Monotub Refill Kit – Sterile Grain Spawn & CVG Substrate

See Reviews

$29.99
$29.99
Sale Sold out
Shipping calculated at checkout.

In stock

View full details

Large Monotub Refill Kit – Clean, Flexible, and Ready to Grow

5 lb Sterile Milo + 5 lb CVG Substrate — Vacuum-Sealed & Built for Bigger Runs

Reload. Scale. Stay Efficient.
Already have your tub and tools? The Large Monotub Refill Kit is a streamlined restock option for growers who just need the essentials. Includes high-quality 5 lb sterile Milo grain and a 5 lb CVG substrate block, vacuum-sealed and prepared for clean handling and consistent performance.

What's Included

  • 5 lb Sterile Milo Grain Bag – Hydrated, pressure-sterilized, sealed with self-healing injection port and filter patch
  • 5 lb CVG Substrate Block – Pasteurized coir/vermiculite/gypsum mix at field capacity

Why Growers Choose This Kit

  • Restock your setup fast — no extra tub or liner required
  • Flexible fruiting: monotub or direct bag fruiting
  • Designed for larger substrate runs
  • Vacuum-sealed freshness with pro-grade prep

Quick Start Guide

Step Instructions
1. Inoculate Clean injection port with 70% isopropyl alcohol. Inject 5–10 mL of liquid culture or spore syringe. Gently shake to distribute.
2. Colonize Store at 70–75°F in a clean, dark area. Once 20–30% colonized, mix to accelerate growth. Full colonization typically takes 10–18 days.
3. Combine Mix colonized grain with the 5 lb CVG substrate. Combine inside a monotub or directly in the bag for bag fruiting. Level the surface gently.
4. Incubate Maintain 70–75°F in indirect light. Allow 5–10 days for full substrate colonization.
5. Initiate Fruiting Introduce light and fresh air. Crack the lid (for tubs) or cut small slits in the bag and cover with micropore tape if needed.
6. Mist & Monitor Mist side walls as needed to maintain humidity. Pins typically appear in 5–7 days once fruiting conditions are introduced. Adjust airflow as needed.

Storage & Shelf Life

  • Store sealed between 45–70°F until use
  • Best used within 15–30 days of receipt
  • Do not freeze; refrigerate fully colonized grain only if necessary
  • Timelines vary depending on genetics and environment
✅ For legal gourmet/culinary/medicinal mushroom cultivation only. This kit is intended exclusively for growing edible gourmet, culinary, and medicinal mushroom species. Not intended for any illegal use.

Don't Forget Your

Replacement Monotub Filters

Apply to your fruiting chamber to give your substrate fresh air while maintaining high humidity levels.

Real Results from Real Growers

4.91 ★ (838)
Verified