No Prep, No Problem: A Guide to Using Our Refill Kits
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From Bag to Bin (or Bag!): How to Use Our Refill Kits in Different Setups

Once you’ve run through a setup or two, you start to realize it’s all about staying stocked, staying clean, and keeping things consistent. That’s where our Refill Kits come in.
Whether you're working with shoeboxes, larger containers, or keeping everything inside the bag — our kits include prepared grain, balanced substrate, and optional agar plates for testing or refining cultures. No prep work, no guesswork — just clean, ready-to-use materials.
Below is a simple overview of how these components are commonly used together.
What’s Included
- Prepared Grain Bags (Milo or Corn)
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Substrate Bags (CVG-based blends)
Step 1: Introduce Your Starting Material
There are a few common ways to begin working with prepared grain:
1. Liquid or Spore Solution
Apply a small amount through the injection port using clean technique and sterilized tools.
2. Agar-Based Transfer
Sections of a clean agar plate can be introduced into the grain in a controlled environment, then sealed immediately.
📌 Tip: Agar work allows for better visibility and consistency before moving forward.
Step 2: Allow the Grain to Develop
Place your grain bag in a clean, stable environment. Over time, you’ll begin to see white growth spreading throughout the grains. Once the material looks consistent and evenly developed, you can move to the next step.
Option 1: Using a Container Setup

- Prepare Your Container: Clean thoroughly and set up your workspace
- Break Up Grain: Gently separate the contents inside the bag
- Combine with Substrate: Mix evenly at your preferred ratio
- Allow to Settle: Let the material stabilize in a consistent environment
- Maintain Conditions: Monitor airflow, moisture, and overall environment
Option 2: Working Directly in the Bag
This method is efficient and works well in limited space or simplified setups.

- Start with Developed Grain: Ensure the grain is evenly prepared
- Combine with Substrate: Add grain to substrate and mix thoroughly
- Level the Material: Flatten to create an even surface
- Allow to Develop: Place in a stable environment
- Adjust Airflow if Needed: Minor adjustments can be made depending on setup
Why Use Our Kits?
- ✅ No prep required – Ready to use
- ✅ Consistent quality – Prepared using controlled processes
- ✅ Small team focused – Built with care and attention to detail
One Last Tip: Label Everything
Keep track of what you’re working with by labeling dates and notes. It makes a big difference when dialing in consistency over time.
Have questions? Reach out anytime — we’re always here to help.
For research and educational purposes only. Intended for use in the study of gourmet and medicinal mushroom species.