• Stacked refill kit components against stylized cosmic background: milo grain bag on top of two CVG substrate blocks.
  • Vacuum‑sealed clear bag of sterile 5 lb milo grain with front injection port resting atop two sealed 10 lb CVG substrate blocks.
  • Close-up of barehands holding moist CVG substrate block, highlighting texture of coco‑coir, vermiculite, gypsum blend.
  • Shot of a 5 lb bag of not so simple substrate, mushroom substrate
  • 5-pound bag of sterile milo (sorghum) grain spawn in Unicorn bag with built-in injection port
1 5

Mycologysimplified

Extra Large Monotub Refill Kit

Extra Large Monotub Refill Kit

See Reviews

$39.99
$39.99
Oferta Agotado
Los gastos de envío se calculan en la pantalla de pago.

En existencias

Ver todos los detalles

Small Monotub Refill Kit – Compact & Clean Growing

3 lb Sterile Milo + 3 lb CVG Substrate — Vacuum-Sealed & Ready to Run

Restock Fast. Keep It Clean.
The Small Monotub Refill Kit makes it easy to reload your setup with high-quality essentials. Includes a 3 lb sterile Milo grain bag and a 3 lb CVG substrate block, both vacuum-sealed and prepped for consistent performance. Fruit in a monotub or directly in the bag — your workflow, your choice.

What’s Included

  • 3 lb Sterile Milo Grain Bag – Hydrated, pressure-sterilized, sealed with self-healing injection port and filter patch
  • 3 lb CVG Substrate Block – Pasteurized coir/vermiculite/gypsum blend, hydrated to field capacity

Why Growers Choose This Kit

  • Perfect for restocking existing monotubs
  • Flexible fruiting options: monotub or in-bag
  • Small-batch prepared for consistency
  • Clean, vacuum-sealed packaging

Quick Start Guide

Step Instructions
1. Inoculate Clean injection port with 70% isopropyl alcohol. Inject 4–10 mL of liquid culture or spore solution. Gently shake to distribute.
2. Colonize Store at 70–75°F in a clean, dark area. Once 30–70% colonized, mix (optional) to accelerate growth. Full colonization typically takes 10–18 days.
3. Combine Mix colonized grain with the 3 lb CVG substrate. Combine inside a monotub or directly in the bag. Gently level the surface.
4. Incubate Maintain 70–75°F in indirect light. Allow 5–10 days for full substrate colonization.
5. Initiate Fruiting Introduce light and fresh air. Crack the tub lid or cut small slits in the bag and cover with micropore tape if needed.
6. Mist & Monitor Mist tub walls or bag interior as needed. Pins typically appear in 5–7 days once fruiting conditions are introduced.

Storage & Shelf Life

  • Store sealed between 45–70°F until ready
  • Best used within 15–30 days of receipt
  • Do not freeze; refrigerate fully colonized grain only if necessary
  • Timeframes vary depending on genetics and environment

Disclaimer

For laboratory use only. Not intended for illegal use. All sales are final, but we’re happy to help with concerns on a case-by-case basis.

Don't Forget Your

Replacement Monotub Filters

Apply to your fruiting chamber to give your substrate fresh air while maintaining high humidity levels.

Let customers speak for us

from 791 reviews
12qt Shoebox Refill Kit | Mushroom Substrate and Grain
B.P.

the grain: i picked milo took off nicely, 2 cc’s per bag, 5 bags per syringe. Works very nice, bags will be done withing a week. I should break and shake right now but, I think ill wait another week or so. As for the bulk substrate, looks great ive been keeping it in our storage room, its super chilly in there. And, it should work great when the time comes. Personally, ive never had issues with any of the bags ive purchased. I hardly inoc in my SAB anymore, as theres no need I just flame sterilize the needle, and inoculate.

12qt Shoebox Refill Kit | Mushroom Substrate and Grain
XLS-B HORIZON UnicornBag - Mushroom Grow Bags - 5 -Micron Gas Exchange Filter Patch
Q.P.

The best , that can be obtained.

XLS-B HORIZON UnicornBag - Mushroom Grow Bags - 5 -Micron Gas Exchange Filter Patch
Syringe Tip Cap Luer Lock (10pk)
Q.P.

They are what they are.

Syringe Tip Cap Luer Lock (10pk)
25 - Sterile 50 mL Conical Tubes with Plastic Rack
Q.P.

Haha I finally got some before they ran out!

25 - Sterile 50 mL Conical Tubes with Plastic Rack
LCD Digital Scales
Q.P.

It works 💪

LCD Digital Scales
2 Pack of 3lb Milo Sorghum Sterile Mushroom Grain Bags
Q.P.

Exactly what i wanted!
Great products!

2 Pack of 3lb Milo Sorghum Sterile Mushroom Grain Bags
2 Pack of 3lb Milo Sorghum Sterile Mushroom Grain Bags
Hector

Absolutely great grains! Everything I have ever purchased from here is amazing and solid 🙌🏽

2 Pack of 3lb Milo Sorghum Sterile Mushroom Grain Bags
6qt Shoebox Refill Kit | Mushroom & Grain Combo
M.L.

Everything arrived safely and packaged very thoughtfully. Grain looks great!

6qt Shoebox Refill Kit | Mushroom & Grain Combo
3lb Mushroom Corn Sterile Grain Bag
Hector

Superb quality as always 👌🏽

3lb Mushroom Corn Sterile Grain Bag
Simple Mushroom Substrate - Ready-to-Use CVG/CVA Mix for Beginners
Melanie

Finally got around to using the substrate and wow, loving my results. Forever gonna be my go to!! Def beats making my own.

Simple Mushroom Substrate - Ready-to-Use CVG/CVA Mix for Beginners
Simple Mushroom Substrate - Ready-to-Use CVG/CVA Mix for Beginners
Kim

Great stuff. 👍

Simple Mushroom Substrate - Ready-to-Use CVG/CVA Mix for Beginners
Pre Poured Sorghum Yeast Agar for Fungi Culturing – Sterile, High-Quality Sorghum Yeast Agar
Emmanouel

Plates looked great! All arrived with no spots of contamination and only one had any condensation on them!

Pre Poured Sorghum Yeast Agar for Fungi Culturing – Sterile, High-Quality Sorghum Yeast Agar
2 Pack of 3lb Milo Sorghum Sterile Mushroom Grain Bags
Emmanouel

Bags arrived intact and packed with care. Signs of growth in just a couple days! So far so good!

2 Pack of 3lb Milo Sorghum Sterile Mushroom Grain Bags
Drippy Corn Mushroom Grain Spawn
Hector

Came packaged vacuum sealed which is great and i really appreciate that, 🙏🏽 smells very clean and everything came shipped way faster than expected, will post another review after results! Highly recommend 👌🏽

Drippy Corn Mushroom Grain Spawn
Simple Mushroom Substrate - Ready-to-Use CVG/CVA Mix for Beginners
Hector

Great moisture content, all in all a really good product cant go wrong here, the myc loves this stuff, truly.

Simple Mushroom Substrate - Ready-to-Use CVG/CVA Mix for Beginners