All In One Bag INSTRUCTIONS

XLS-A Unicorn mushroom bag

Unicorn Bag

Material: 3.0 Mil Polypropylene

Filter Pore Size: 0.2 microns

Small injection port over a grain bag

Injection Port

Injection port: Thermally bonded into the bag, no additional sealing necessary

XLS-A Unicorn mushroom bag

Unicorn Bag

Material: 3.0 Mil Polypropylene

Filter Pore Size: 0.2 microns

Small injection port over a grain bag

Injection Port

Injection port: Thermally bonded into the bag, no additional sealing necessary

Close-up of Substrate material with a blurred background

Substrate

2.5 Pounds: CVG/A

Properly Hydrated: For optimal growth

Close-up of brown and beige quinoa seeds

Grain

2.5 Pounds

Hydrated: Ideal moisture level

Sterilized: Profesional Sterilization

AIO Bag on white background
Close-up of Substrate material with a blurred background

Substrate

2.5 Pounds: CVG/A

Properly Hydrated: For optimal growth

Close-up of brown and beige quinoa seeds

Grain

2.5 Pounds

Hydrated: Ideal moisture level

Sterilized: Profesional Sterilization

Jump to FAQ ↓
Quick answers for troubleshooting and best results.
Before You Start
This guide is here as a reference — not something that has to be followed exactly step-by-step. There are many ways to use an all-in-one bag, and what you’ll find here covers the basic process along with a few variations for fruiting. The timelines listed are just averages and can be shorter or longer depending on your environment, conditions, and genetics.
Illustration of a hand wearing a glove cleaning a surface with a spray bottle labeled '70%'.

Prepare Your Workspace

SANITIZE

Choose a clean area with minimal airflow (no fans, vents, or open windows).

Sanitize thoroughly:

Sanitize Thoroughly

  1. Put on the included gloves, then wipe them down using one of the included 70% isopropyl alcohol wipes.
  2. Wipe down your work surface thoroughly with 70% isopropyl alcohol.

Prepare the Bag

  1. Remove the All-In-One bag from the secondary vacuum packaging.
  2. If the bag appears compressed from the vacuum seal, gently lift the bag flap near the filter patch and allow a small amount of air to enter the bag. This lets the filter patch equalize pressure and gives the bag room to breathe.


*Cleanliness is the #1 factor in preventing contamination.

Illustration of a transparent bag with a syringe injecting a substance, on a beige background.

Inoculate Your Grow Bag

INOCULATION

You will need your own liquid culture or spore syringe.

  1. Wipe the injection port with an alcohol pad before inserting the needle.
  2. Flame-sterilize your needle until it glows red. Let the needle cool for ~3 seconds — do not touch or wipe the needle after flaming.
  3. Insert the needle straight through the self-healing injection port.
  4. Inject the appropriate amount:
  5. Withdraw the needle and recap.

Liquid Culture (LC):
• 5 lb bag: 2–10 cc
• 3 lb bag: 2–3 cc

Spores (MSS): Use at least 5 cc.

*After inoculation, place the bag in a dark or low-light area at 70–75°F (21–24°C). Avoid direct sunlight.

*Mycelium growth is usually visible within 5–10 days depending on genetics and conditions.

Colonized substrate

Be Patient

COLONIZATION

Mycelium will spread outward from the injection point.

What you’ll see:

  • 0% – plain grain
  • 25% – small white patches
  • 50% – roughly half white
  • 100% – completely white, no bare grain

Be Patient - Avoid handling the bag constantly. Mycelium forms tiny fibers that can break if the bag is repeatedly moved or squeezed.

Full colonization typically takes 14–28 days, depending on genetics, temperature, and your environment, though times may vary.

*Do not mix the bag until the grain is about 50–80% colonized.

Illustration of hands holding a package with a light bulb on a beige background

Mix Grain Into Substrate

BREAK AND SHAKE

When the grain portion is 50–80%% colonized:

  1. Break up the colonized grain again.
  2. Mix it thoroughly into the substrate layer.
  3. Let the contents settle to the bottom.
  4. Lightly compress the block.

Return the bag to a dark area at 70–75°F for another 2–3 weeks, or until the entire block becomes solid white.

Illustration of a clear bag with mushrooms on a metal surface.

Fruiting Setup

INITIATE FRUITING

Once the block is fully colonized (completely white with no exposed substrate):

Move the bag to a fruiting area that provides:

  • 8–12 hours of indirect light each day
  • Light in the 5000–6000K color range
  • Temperatures between 60–75°F

Top-Fruiting (Recommended Method)

  1. Cut a small slit in bag with a sterile blade and cover with micropore tape.
  2. Carefully pull on sides of bag to pull air inside through the new opening.
  3. Maintain proper humidity — light condensation inside the bag is ideal.

Pins typically begin forming within 1–2 weeks.

Illustration of a container and a bag with mushrooms on a beige background

Alternative Fruiting Methods

OPTIONAL METHODS

A- Cut the bag

  1. Slightly open the top of the bag to introduce fresh air.
  2. Close it loosely so the bag stays inflated.
  3. Maintain humidity — you should see light condensation on the inside of the bag.

B - Do Nothing Method

  1. Slowest: 6–8 weeks
  2. Zero maintenance
  3. Some genetics fruit extremely well this way.

C - Remove Bag & Fruit as a Cake

  1. Remove the bag and place the block inside a tote or monotub.
  2. Fan fresh air 1-2× daily.
Illustration of a hand holding scissors to open a bag of mushrooms on a beige background

Twist and Pull

HARVESTING

Harvest when the veil under the mushroom cap begins to tear.

  1. Cut the top of the bag fully open.
  2. Twist mushrooms gently at the base to remove them.
  3. For a second flush:
  • Leave the block inside the bag
  • Mist lightly to rehydrate
  • Keep the top loosely open

Most growers get:

  • 1 large flush
  • Optional smaller 2nd flush

Discard immediately if you see:

  • Green, black, pink, or bright yellow mold
  • Slime or foul odor
  • Strange discoloration
Illustration of a jar with mushrooms and environmental factors affecting their growth.

Environmental Guidelines

ENVIRONMENT

LIGHT – Indirect, 8–12 hours/day
FRESH AIR – Loosen or open the bag slightly
HUMIDITY – Light moisture should stay on the bag walls
TEMPERATURE – 65–75°F

  • Too warm = contamination risk
  • Too cold = very slow growth
Illustration of four bags labeled 'Overly Dry', 'Too Wet', 'Not Enough Air', and 'Contamination' on a beige background.

Diagnostics

TROUBLESHOOTING

No growth:
Spores can take 3+ weeks. LC is faster and more reliable.

Tall/fuzzy/skinny mushrooms:
Increase fresh air exchange. With a clean scalpel cut a small 1”-2” slit just below the filter patch and cover with micropore tape. 

Dry or cracked caps:
Increase humidity.

Pale mushrooms:
Give more indirect light.

Contamination:
Dispose of the bag and start fresh.

Frequently Asked Questions

Setup Do I Need Special Equipment? +
No special equipment is required. These bags are prepared and ready to use, making them a simple option for beginners or anyone looking for a low-maintenance setup.
Environment What Temperature Is Best? +
A stable environment between 68–75°F is ideal. Consistency matters more than warmth—avoid large temperature swings or direct heat sources.
Light Do I Need Light? +
Ambient room light is enough. Avoid direct sunlight, as it can overheat and dry the bag.
Storage Can I Store the Bag Before Use? +
Yes. Keep it sealed in a clean, room-temperature area away from direct sunlight. Avoid extreme heat, cold, or humidity.
Moisture Is Condensation Normal? +
Yes. Light condensation or small droplets are normal, especially with temperature changes. Stable conditions help reduce excess moisture.
Timing How Long Until I See Progress? +
Timing can vary depending on conditions, but most users begin to see early signs of progress within several days to a couple of weeks.
Handling Should I Move or Adjust the Bag Often? +
It’s best to keep handling to a minimum. A stable, undisturbed environment usually leads to better results.
Quality What If Something Looks Off? +
If you notice unusual colors, strong odors, or anything that seems off, isolate the bag and reach out for support. We’re happy to help.
Tip: Stable conditions and clean handling go a long way.

Ready for Your Next Grow?

We carry everything you need to keep things moving.

• Sterile Grain Bags • Substrates • Pre-Poured Agar Plates • Unicorn Grow Bags • Lab Supplies • Grow Kits & Refills